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CleanCap® FLuc mRNA (5moU)

SKU: L-7202

Description

The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

This mRNA is capped using CleanCap® Reagent AG, TriLink's patented co-transciptional capping technology, which results in the naturally occurring Cap-1 structure with >95% capping efficiency. It is manufactured using TriLink's CleanScript™ method*, polyadenylated, modified with 5-methoxyuridine, and optimized for mammalian systems. It mimics a fully processed mature mRNA.

CleanCap® Reagent AG produces a base-modified Cap-1 mRNA, which shows superior in vivo activity compared to Cap-0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).

5-methoxyuridine is a modified uridine that can reduce immunogenic response and enhance translational efficiency of mRNAs. These properties can result in safer mRNA and increased protein expression.

CleanScript™ method is TriLink's proprietary in vitro transcription process that is optimized to minimize dsRNA formation and increase mRNA activity in vivo.

*Starting April 30th, 2024

Product details
Catalog No L-7202
Purity Passes Agarose Gel Mobility
Concentration 1.0 mg/mL
Buffer 1 mM Sodium Citrate pH 6.4
Recommended Storage At or below -40°C
Application Reporter Genes
Base Composition Fully substituted with 5-Methoxy-U
Brand CleanCap
Cap AG Start
Composition Fully substituted with 5-Methoxy-U
Conversion Factor 40 µg/OD₂₆₀
length 1922 nucleotides
Phosphatase Treated True
Shipping Temp Frozen

Technical documents

Certificate of analysis

Intellectual property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

CleanCap capping technology

For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. For additional licensing restrictions, please see the license agreement at trilinkbiotech.com/cleancap-research-license. Patents and patent pending, see trilinkbiotech.com/legal-notices.

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