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CleanCap® AG 3′ OMe CleanScript™ IVT Kit and mRNA ModTail™ Kit Bundle

SKU: K-9613

Description

TriLink’s CleanCap® AG 3′ OMe CleanScript™ IVT Kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 20 x 20 µL or 125 x 20 µL reactions depending on the kit size and following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes, a reaction buffer, and a control DNA template. Each 20 µL reaction typically produces 160–200 µg of capped mRNA, corresponding to approximately 3.2–4 mg per 20-reaction kit (K-7413-20) and 20–25 mg per 125-reaction kit (K-7413-125).

TriLink’s mRNA ModTail™ Kit supplies our proprietary reagent and enzyme mixes for the installation of a poly(A) tail modification on purified mRNA. The kit components are sufficient to convert 2.5 mg of purified mRNA to ModTail™ mRNA following our included protocol. 

Both CleanCap® IVT Kit and mRNA ModTail™ Kit can be used independently as well.

The CleanCap® AG 3′ OMe CleanScript™ IVT kit is designed for cumulative gains in yield, quality, and expression. Key benefits of TriLink’s IVT kits compared to others with enzymatic or other co-transcriptional capping include:

  • Time and cost savings - Co-transcriptional capping protocol with all necessary reagents for a single-tube reaction providing high yield, fewer steps, and less handling
  • High capping efficiency - Protocol with CleanCap AG 3′ OMe cap analog to produce an optimal cap 1 with over 95% capping efficiency
  • Increased mRNA yield - Buffer and kit components formulated using TriLink’s proprietary CleanScript IVT method for up to 10 mg/mL yield
  • Ultra-low double-stranded RNA (dsRNA) levels - Up to 85% dsRNA reduction using CleanScribe™ RNA Polymerase, in place of wild-type T7 RNA polymerase in combination with the CleanScript IVT method (instead of standard IVT)
  • Enhanced mRNA performance - Robust protein expression and low inflammatory responses from mRNA synthesized with CleanCap AG 3′ OMe, CleanScript method, CleanScribe RNA polymerase, and N1-methylpseudouridine

ModTail™ kit’s key features and benefits include: 

  • Enhanced protein expression and duration - ModTail™ technology increases mRNA stability and protein expression in vivo 
  • High integration efficiency - the protocol results in ≥90% integration efficiency for most constructs 
  • Broad compatibility - with most mRNA constructs regardless of cap analog, base modification, or tail length 
  • Easy to use - No change to delivery process or impact on critical quality attributes such as integrity, capping, and dsRNA

This bundle includes:

Important note:

  • Please refer to the individual product webpages above for kit specific information and FAQs.

Product Attributes
Catalog No. K-7413
Application In vitro Transcription, co-transcriptional capping, mRNA synthesis
Cap AG Start, Cap 1
Cap Analogs CleanCap® AG 3’OMe
Product Grade RUO
Reaction Size 20 x 20 µL (K-7413-20)
125 x 20 µL (K-7413-125)
Recommended Storage -15°C to -25°C 

mRNA ModTail™ Kit
Catalog No. K-2200-S
Application enhanced protein expression and duration, CRISPR/Car-T, RNA therapeutics  
Components ModTail™ Enzyme Mix and ModTail™ Reaction  Mix 
Product type Poly(A) tail modification kit  
Reaction size Converts up to 2.5 mg of purified mRNA  
Recommended Storage -15°C to -25°C  

Technical documents

Certificate of analysis

Intellectual property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

CleanCap capping technology

For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. For additional licensing restrictions, please see the license agreement at trilinkbiotech.com/cleancap-research-license. Patents and patent pending, see trilinkbiotech.com/legal-notices.

ModTail™ technology

For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. For additional licensing restrictions, please see the license agreement at trilinkbiotech.com/modtail-research-license. Patents and patents pending, see (trilinkbiotech.com/legal-notices)[https://trilinkbiotech.com/legal-notices].

  • CleanCap® AG 3′ OMe CleanScript™ IVT Kit and mRNA ModTail™ Kit Bundle (sold as Each)
    SKU: K-9613-20S
    $880.00
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