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BsaI

SKU: E-0099

Description

Bsal is a type IIS restriction enzyme that recognizes a specific DNA sequence (5′-GGTCTC-3′) and cleaves DNA outside of this recognition site, typically leaving 4-base overhangs.

BsaI’s recognition and cleavage sites are:
5′ GGTCTCN↓ 3′
3′ CCAGAGNNNNN↑ 5′

BsaI is thermostable and can be used in various molecular biology applications, including generation of linear DNA templates for in vitro transcription to synthesize RNA. Type IIS restriction enzymes bind to asymmetric DNA recognition sites but cut the DNA at defined positions located outside of those sites.

The enzyme is provided 20 U/μL in storage buffer. It is also supplied with 1.25 mL of 10X RE Reaction Buffer (500 mM Tris, 1000 mM NaCl, 100 mM MgCl2, 1 mg/mL rHSA, pH 7.9 at 25°C).

Unit definition: The amount of BsaI required to digest 1 µg of pXba DNA in 1 hour at 37°C in a 50 µL reaction.

Product details
Catalog No E-0099
Concentration 20 U/µL
Buffer 10 mM Tris, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5 g/L rHSA, 50% glycerol, pH 7.5 at 25°C
Volume 50 µL, 250 µL
Host E. coli
Recommended Storage -15 to -25°C
Application IVT template preparation, restriction digestion
Product Grade Molecular Biology
Shipping Temp Frozen
Size 1,000 U, 5,000 U

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