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CleanScribe® RNA Polymerase

SKU: E-0107

Description

TriLink’s CleanScribe™ RNA Polymerase is a novel DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. This enzyme reduces double-strand RNA (dsRNA) levels by up to 85% during IVT. dsRNA is a byproduct of IVT reactions and can trigger undesirable inflammatory responses in host cells. This novel RNA polymerase can directly substitute a wild-type T7 RNA polymerase in an IVT protocol to synthesize RNAs from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its robustness, reproducibility, and dsRNA reduction make it ideal for RNA synthesis as well as other applications such as radiolabeled RNA probe preparation and RNA construct development for additional studies. E-0107-001, -01, and -08 are provided at a 2X higher concentration than E-0107-S and -L for specialized applications and CleanCap® co-transcriptional capping of RNA constructs following TriLink’s protocols.  E-0107-C is supplied at a 10X higher concentration than E-0107-S and -L, and it includes 10 mL of dilution buffer to facilitate the optimization of reaction parameters.
Cat number Concentration Intended use
E-0107-S, E-0107-L 50,000 U/mL Traditional protocols
E-0107-001, E-0107-01, E-0107-08 100,000 U/mL TriLink protocols and specialized applications
E-0107-10 500,000 U/mL Protocol development

Unit definition: One unit of enzyme incorporates 1 nmol of ATP in 1 hour at 37°C.

Product details
Catalog No E-0107
Concentration 50 U/μL, 100 U/μL, 500 U/μL
Buffer 50mM Tris, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 50% glycerol, 0.1% Tween-20, pH 8.0
Volume 0.05 mL, 0.1 mL, 0.5 mL, 4.2 mL, 1.0 mL
Host E. coli
Recommended Storage -15 to -25°C
Product Grade Molecular biology
Reaction Size 25

Technical documents

Product FAQs

ISO 13485 compliant.

Our CleanScribe RNA Polymerase uses the same promoter sequence as a wild-type T7 RNA polymerase, but is not a T7 enzyme.

We are characterizing CleanScribe RNA Polymerase's performance across a variety of IVT conditions. It shows promising results, and we expect the enzyme to perform well under your conditions.

Yes

Yes, if you don't linearize, the transcription will continue multiple times and you will not get the expected mRNA product.

Certificate of analysis

Intellectual property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

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