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Inorganic Pyrophosphatase (iPPase)

SKU: E-0051

Description

E. coli inorganic pyrophosphatase (iPPase) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to form orthophosphate. Using iPPase in an in vitro transcription reaction drives RNA synthesis forward by removing pyrophosphate which forms as a byproduct and precipitates with key transcription buffer cations.

The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert. The 30 µg and 310 µg pack sizes contain approximately 6 units and 62 units of enzymes, respectively.

Unit definition: One unit of enzyme produces 1 µmol of phosphate from inorganic pyrophosphate in 1 min at 25°C.

Product details


Catalog No	E-0051
Concentration	0.5 mg/mL
Buffer	20 mM Tris
Volume	60μL
Host	E. coli
Recommended Storage	-20 to -25°C
Reaction Size	25.0

Technical documents

Safety Data Sheet Look-up
E-0051 Product Insert

Product FAQs

ISO 13485 compliant.

Typically, we recommend 0.002U/mL.

Certificate of analysis

Certificate of Analysis (CoA)

Intellectual property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

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