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T7 RNA Polymerase

SKU: E-0053

Description

Wild-type bacteriophage T7 RNA polymerase is a highly processive, 100 kDa, DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. It is a key enzyme used to synthesize RNA from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its high robustness and reproducibility make it the standard industry choice in IVT as well as other applications such as radiolabeled RNA probe preparation and RNA construct development for additional studies. E-0053-001, -01, and -08 are provided at a 2X higher concentration than E-0053-S and -L for specialized applications and CleanCap® co-transcriptional capping of RNA constructs.
Catalog number Concentration Intended Use
E-0053-S, E0053-L 50,000 U/mL Traditional protocols
E-0053-001, E-0053-01, E-0053-08 100,000 U/mL TriLink protocols and specialized applications

Unit definition: One unit of enzyme incorporates 1 nmol of ATP in 1 hour at 37°C.

Product details
Catalog No E-0053
Concentration 50 U/μL, 100 U/μL
Buffer 50mM Tris, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 50% glycerol, 0.1% Tween-20, pH 8.0
Volume 0.05 mL, 0.1 mL, 0.5 mL, 4.2 mL
Host E. coli
Recommended Storage -20 to -25°C
Reaction Size 25

Technical documents

Product FAQs

No, this is a standard wild type with no modifications.

Yes

ISO 13485 compliant.

The yield is dependent on the IVT conditions (pulse vs traditional IVT) as well as inputs such as Cap, NTPs and modified NTP concentration as well as the amount of DNA template, and Polymerase.

Yes, if you don't linearize, the transcription will continue multiple times and you will not get the expected mRNA product.

Typically, it may be due to RNase contamination, as well as salt concentration and pH.

Certificate of analysis

Intellectual property

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