T7 RNA Polymerase
Description
Wild-type bacteriophage T7 RNA polymerase is a highly processive, 100 kDa, DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. It is a key enzyme used to synthesize mRNA (messenger RNA) from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its high robustness and reproducibility makes it the standard industry choice for mRNA synthesis as well as other applications such as saRNA synthesis, radiolabeled RNA probe preparation, and RNA construct development for additional studies.
The provided pack sizes are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our recommended co-transcriptional protocol to produce Cap-1 mRNAs. Please see the details in the product insert. The 100 µg and 840 µg pack sizes contain approximately 50,000 units and 420,000 units of enzymes, respectively.
Unit definition: One unit of enzyme incorporates 1 nmol of ATP in 1 hour at 37°C.
Product details
| Catalog No | E-0053 |
| Concentration | 0.2 mg/mL |
| Buffer | 50mM Tris |
| Volume | 0.5 mL |
| Host | E. coli |
| Recommended Storage | -20 to -25°C |
| Reaction Size | 25.0 |
Technical documents
- Safety Data Sheet Look-up open_in_new
- E-0053 Product Insert open_in_new
Product FAQs
No, this is a standard wild type with no modifications.
Yes
ISO 13485 compliant.
The yield is dependent on the IVT conditions (pulse vs traditional IVT) as well as inputs such as Cap, NTPs and modified NTP concentration as well as the amount of DNA template, and Polymerase.
Yes, if you don't linearize, the transcription will continue multiple times and you will not get the expected mRNA product.
Typically, it may be due to RNase contamination, as well as salt concentration and pH.
Certificate of analysis
- Certificate of Analysis (CoA) open_in_new
Intellectual property
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.