Biotin-16-Aminoallyluridine-5'-Triphosphate
Description
Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA. According to Fenn BJ, et al., T7 RNA polymerase does not significantly discriminate between the natural and the biotinylated nucleotide. Therefore, the number of biotinylated positions per transcript can be controlled by varying the ratio of the biotinylated NTP to the standard NTP. Biotinylated RNA probes are useful as a nonradioactive label in a number of applications including chromosome mapping, in situ hybridization and microarray hybridization. Biotin-16-U-modified RNA can be detected with a variety of streptavidin-based approaches.
Product details
| Catalog No | N-5005 |
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 7 |
| Molecular Formula | C₃₂H₅₂N₇O₁₉P₃S (free acid) |
| Molecular Weight | 963.80 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 10 mM |
| Buffer | H₂O |
| Recommended Storage | -20°C or below |
| Application | Aptamers |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Uridine |
| Nucleotide Category | Biotin Labeled |
| Shipping Temp | Frozen |
| Sugars | RNA |
Technical documents
- Safety Data Sheet Look-up open_in_new
Certificate of analysis
- Certificate of Analysis (CoA) open_in_new
Intellectual property
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.
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