Guanosine-5'-Triphosphate
Description
Adenosine-5'-triphosphate (ATP), cytidine-5'-triphosphate (CTP), guanosine-5'-triphosphate (GTP), and uridine-5'-triphosphate (UTP) are unmodified ribonucleoside triphosphates, serving as essential building blocks of RNA molecules. Each of them consists of an unmodified base, a ribose, and a 5' triphosphate group. They can be used with modified nucleotides and TriLink's patented CleanCap® capping technology in in vitro transcription for robust RNA manufacturing. TriLink offers both research-use-only (RUO) and good manufacturing practice (GMP) rNTPs. TriLink's GMP rNTPs are manufactured in highly controlled environments with documented procedures, traceability of materials and processes, and strict quality control measures, providing exceptional consistency, purity, and safety. Analytical procedures performed on TriLink's nucleotides for quality assurance include:
| RUO products | GMP products (in addition RUO analyses) |
|---|---|
| • pH | • Characterization: appearance, residual chemicals |
| • Concentration: UV/Vis | • Safety: endotoxin, bioburden |
| • Purity: 31P NMR, AX-HPLC | • Nuclease contamination: DNase and RNase detection |
| • Identity: 1H NMR, mass spectrometry |
The rNTPs are provided 100 mM in H₂O, pH 7.5.
Product details
| Catalog No | N-1503 |
| Purity | ≥99% by AX-HPLC |
| Extinction Coefficient | 13 |
| Molecular Formula | C₁₀H₁₆N₅O₁₄P₃ (free acid) |
| Molecular Weight | 523.10 g/mole (free acid) |
| Salt Form | Na+ |
| Concentration | 100 mM |
| Buffer | H₂O |
| Recommended Storage | -20°C or below |
| Application | Aptamers, In vitro Transcription, Laboratory Developed Tests (LDT) & Analyte Specific Reagents (ASR), PCR & RT-PCR |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Guanosine |
| Nucleotide Category | Unmodified |
| Ph | 7.5±0.5 |
| Shipping Temp | Frozen |
| Sugars | RNA |
Technical documents
- Safety Data Sheet Look-up open_in_new
Product FAQs
A nucleoside contains only the base and the sugar (5'-OH) while a nucleotide refers to a nucleoside with phosphorylation on the sugar. This includes the phosphate groups on the 2', 3' or 5' ends.
A common approach is to use nucleotides with a 2_-modified ribose such as 2_- fluoro or 2_-o-methyl.
They are resuspended in water, pH 7.5±0.5.
A 1 µmole vial of 100 mM NTP contains 10 µL.
In general, we recommend equimolar amounts of nucleotides in IVT. The exact amounts depend on the IVT reagents and conditions. Please see our rNTP product inserts for IVT with CleanCap M6 and CleanCap AU analogs.
Yes, it can. Our product inserts have recommended ratios for small-scale IVT. If you would like to discuss it further, please contact us at trilinkbiotech.com/contact-us or support@trilinkbiotech.com.
When stored properly at or below -20°C, NTPs should be stable for a few years. Upon first use, prepare aliquots to minimize freeze-thaw cycles and NTP degradation.
Although NTPs are stable at room temperature for several days, they are shipped on wet or dry ice to ensure stability in the event of a transportation delay. Even if they have arrived thawed, they should still be good to use. If you have concerns or questions however, please contact us at trilinkbiotech.com/contact-us or support@trilinkbiotech.com.
Each NTP is accompanied by a certificate of analysis with the methods of analysis, concentration, purity, identity, etc. Our GMP NTPs undergo further analyses for characterization, safety, and nuclease contamination.
We run 31P NMR, which reveals inorganic phosphate salt. Enzymologists usually have the most concern about this salt since it inhibits many polymerases.
We provide custom chemistry services. Please inquire about the feasibility of your desired nucleotide at trilinkbiotech.com/custom-chemistry-services.
Certificate of analysis
- Certificate of Analysis (CoA) open_in_new
Intellectual property
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