arrow_back Back

N1-Methylpseudouridine-5'-Triphosphate

SKU: N-1081

Description

N1-Methylpseudouridine-5'-Triphosphate (N1MePsU) is a modified NTP for incorporation into messenger RNAs (mRNA) using an enzyme such as T7 RNA Polymerase. Modified uridines such as N1-methylpseudouridine, 5-methoxyuridine, and pseudouridine can reduce unwanted immunogenic response and enhance translational efficiency of mRNAs. These properties can result in safer mRNA and increased protein expression.  TriLink offers both research-use-only (RUO) and good manufacturing practice (GMP) N1MePsU. TriLink’s GMP rNTPs are manufactured in highly controlled environments with documented procedures, traceability of materials and processes, and strict quality control measures, providing exceptional consistency, purity, and safety.    Analytical procedures performed on TriLink’s nucleotides for quality assurance include:   RUO products   pH Concentration: UV/Vis   Purity: 31P NMR, AX-HPLC   Identity: 1H NMR, mass spectrometry  GMP products (in addition RUO analyses)  Characterization: appearance, residual chemicals  Safety: endotoxin, bioburden  Nuclease contamination: DNase and RNase detection   TriLink’s N1MePsU is provided 100 mM in H2O, pH 7.5. To learn more about additional modified nucleotides, please visit https://www.trilinkbiotech.com/modified-nucleotides  

Product details
Catalog No N-1081
Purity ≥99% by AX-HPLC
Extinction Coefficient 8
Molecular Formula C₁₀H₁₇N₂O₁₅P₃ (free acid)
Molecular Weight 498.10 g/mole (free acid)
Salt Form Na+
Concentration 100 mM
Buffer H₂O
Recommended Storage -20°C or below
Application In vitro Transcription
Backbone 5'-Triphosphate
Base Analog(s) Pseudouridine
Nucleotide Category Base Modified RNA
Shipping Temp Frozen
Sugars RNA

Technical documents

Product FAQs

A nucleoside contains only the base and the sugar (5'-OH) while a nucleotide refers to a nucleoside with phosphorylation on the sugar. This includes the phosphate groups on the 2', 3' or 5' ends.

They are resuspended in water, pH 7.5±0.5.

A 1 µmole vial of 100 mM NTP contains 10 µL.

In general, we recommend equimolar amounts of nucleotides in IVT. The exact amounts depend on the IVT reagents and conditions. Please see our rNTP product inserts for IVT with CleanCap M6 and CleanCap AU analogs.

Yes, it can. Our product inserts have recommended ratios for small-scale IVT. If you would like to discuss it further, please contact us at trilinkbiotech.com/contact-us or support@trilinkbiotech.com.

When stored properly at or below -20°C, NTPs should be stable for a few years. Upon first use, prepare aliquots to minimize freeze-thaw cycles and NTP degradation.

Although NTPs are stable at room temperature for several days, they are shipped on wet or dry ice to ensure stability in the event of a transportation delay. Even if they have arrived thawed, they should still be good to use. If you have concerns or questions however, please contact us at trilinkbiotech.com/contact-us or support@trilinkbiotech.com.

Each NTP is accompanied by a certificate of analysis with the methods of analysis, concentration, purity, identity, etc. Our GMP NTPs undergo further analyses for characterization, safety, and nuclease contamination.

We run 31P NMR, which reveals inorganic phosphate salt. Enzymologists usually have the most concern about this salt since it inhibits many polymerases.

We provide custom chemistry services. Please inquire about the feasibility of your desired nucleotide at trilinkbiotech.com/custom-chemistry-services.

Certificate of analysis

Intellectual property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

support Contact us help Learn more about N1-Methylpseudouridine-5'-Triphosphate