Thienoguanosine-5'-Triphosphate
Description
GTP with a thieno[3,4-d]pyrimidine core (Thieno-GTP) is a fluorescent nucleotide that is readily accepted by T7 polymerase as a faithful GTP surrogate. This highly isomorphic nucleoside triphosphate is able to initiate transcription and incorporate during elongation. Transcripts containing a 5' pppthG are successfully dephosphorylated with alkaline phosphatase and subsequently phosphorylated with T4 polynucleotide kinase, illustrating that other terminus-modifying enzymes tolerate this modification as well.
Importantly, emissive messenger RNAs, substituted with Thieno-G are recognized by the ribosome and are capable of fluorescently reporting discrete steps in real time during translation. Additionally, Thieno-GTP can be utilized to visualize mRNA localization and assess mRNA delivery independent of translation.
Product details
| Catalog No | N-1088 |
| Purity | ≥95% by AX-HPLC |
| Extinction Coefficient | 4 |
| Molecular Formula | C₁₁H₁₆N₃O₁₄P₃S (free acid) |
| Molecular Weight | 539.20 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H₂O |
| Recommended Storage | -20°C or below |
| Application | Aptamers |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Guanosine |
| Nucleotide Category | Base Modified RNA |
| Shipping Temp | Frozen |
| Sugars | RNA |
Technical documents
- Safety Data Sheet Look-up open_in_new
Certificate of analysis
- Certificate of Analysis (CoA) open_in_new
Intellectual property
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