mCAP
Description
Eukaryotic and viral messenger RNA (mRNA) has a monomethylated cap structure, m7G(5')ppp(5')G or mCAP, at the 5'-terminus. The cap analog plays an important role in cellular processes, most notably the initiation of mRNA translation. The cap interacts with different binding proteins, initiation factors and elements of ribosomes. mCAP was the first cap analog to be introduced to the molecular biology toolbox. Half of the time, mCAP inserts in the correct orientation to enhance translation. The other 50% of molecules are not substrates for efficient translation, reducing the specific activity of the transcript. More recently, Anti Reverse Cap Analog (ARCA) was introduced. ARCA can only insert in the proper orientation, resulting in mRNAs that are translated twice as efficiently as those initiated with mCAP. mCAP is incorporated in a transcription by including a mixture of the cap analog and GTP (usually at a 4:1 ratio). Approximately 80% of synthesized mRNA will possess a 5' cap, while the remaining 20% will possess a 5' triphosphate.
Product details
| Catalog No | N-7001 |
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 21 |
| Molecular Formula | C₂₁H₃₀N₁₀O₁₈P₃ (free acid) |
| Molecular Weight | 803.40 g/mole (free acid) |
| Salt Form | NH4+ |
| Concentration | 100 mM |
| Buffer | H₂O |
| Recommended Storage | -20°C or below |
| Application | CRISPR |
| Backbone | 5'-5'-Triphosphate |
| Base Analog(s) | Guanosine |
| Nucleotide Category | Cap analogs |
| Cap Analogs | Other |
| Shipping Temp | Frozen |
Technical documents
- Safety Data Sheet Look-up open_in_new
Certificate of analysis
- Certificate of Analysis (CoA) open_in_new
Intellectual property
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