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mRNA ModTail™ Kit

SKU: K-2200

Description

TriLink’s mRNA ModTail™ Kit supplies our proprietary reagent and enzyme mixes for the installation of a poly(A) tail modification on purified mRNA. The kit components are sufficient to convert 2.5 mg or 15 mg of purified mRNA to ModTail™ mRNA following our included protocol.

Our kit’s key features and benefits include:

  • Enhanced protein expression and duration - ModTail™ technology increases mRNA stability and protein expression in vivo
  • High integration efficiency – the protocol results in ≥90% integration efficiency for most constructs
  • Broad compatibility - with most mRNA constructs regardless of cap analog, base modification, or tail length
  • Easy to use – No change to delivery process or impact on critical quality attributes such as integrity, capping, and dsRNA

The kit contains sufficient quantities of the following components to support the specified number of reactions:

  • ModTail™ Enzyme Mix
  • ModTail™ Reaction Mix

Important note:

  • This kit is specifically formulated for use with purified mRNA that includes a poly(A) tail, is ≤7.5kb, and is stored in water or 1 mM Sodium Citrate pH 6.4
  • The efficiency of the integration is significantly affected by the quality of the mRNA going into the reaction. Post reaction yields will vary depending on the type of purification and amount of recovery from the purification after the ModTail reaction. Please contact [email protected] with any questions.

Product attributes
Application enhanced protein expression and duration, CRISPR/Car-T, RNA therapeutics
Catalog No K-2200
Components ModTail™ Enzyme Mix and ModTail™ Reaction Mix
Product type Poly(A) tail modification kit
Reaction size Converts up to 2.5 mg of purified mRNA (K-2200-S)
Converts up to 15 mg of purified mRNA (K-2200-L)
Recommended Storage -15°C to -25°C

Technical documents

Product FAQs

TriLink’s ModTail™ mRNAs should be compatible with commonly used delivery vehicles. We have used it with common transfection reagents and LNPs, including Lipofectamine™ MessengerMAX™ reagents, and ALC-0315, DSPC, Cholesterol, and DMG-PEG 2000: Pfizer/Acuitas Lipids.

No, we have data showing that ModTail™ mRNAs are of comparable integrity to regular oligo-dT-purified mRNAs.

We have not observed meaningful differences in the immunogenicity of tail-modified mRNA from non-tail-modified mRNA in our in vitro and in vivo studies.

Certificate of analysis

Intellectual property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

ModTail™ technology

For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. For additional licensing restrictions, please see the license agreement at trilinkbiotech.com/modtail-research-license. Patents and patents pending, see (trilinkbiotech.com/legal-notices)[https://trilinkbiotech.com/legal-notices].

  • mRNA ModTail™ Kit, 2.5mg (sold as Each)
    SKU: K-2200-S
    $450.00
  • mRNA ModTail™ Kit, 15mg (sold as Each)
    SKU: K-2200-L
    $1,500.00
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