2',3'-Dideoxythymidine-5'-Triphosphate
Description
2',3'-Dideoxythymidine-5'-Triphosphate (ddTTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3' end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. "I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results." Michal Legiewicz Research Scientist Yale University
Product details
| Catalog No | N-4004 |
| Purity | ≥95% by AX-HPLC |
| Extinction Coefficient | 9 |
| Molecular Formula | C₁₀H₁₇N₂O₁₃P₃ (free acid) |
| Molecular Weight | 466.10 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H₂O |
| Recommended Storage | -20°C or below |
| Application | Aptamers |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Thymidine |
| Nucleotide Category | Sugar Modified |
| Shipping Temp | Frozen |
Technical documents
- Safety Data Sheet Look-up open_in_new
Certificate of analysis
- Certificate of Analysis (CoA) open_in_new
Intellectual property
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.