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ContrOligo™ PolyA RNA Standard, 100A

SKU: O-5001-100A

Description

The ContrOligo PolyA RNA Standard (100A) is a chemically synthesized, 100-nucleotide poly(A) RNA oligonucleotide designed for use as an analytical reference standard. Produced by TriLink BioTechnologies with >30 years of oligo manufacturing expertise, this standard provides a reliable size marker for HPLC , mass spectrometry, and fragment analyzer workflows used in RNA analysis.

Key features of this standard include:

  • 100-nucleotide poly(A) sequence with no exogenous bases
  • Lyophilized format for stability and convenience; simply reconstitute before use
  • Available in 2 nmole (ideal for mass spectrometry or fragment analyzer) and 10 nmole (ideal for HPLC) formats
  • Off-the-shelf availability eliminates the 20–30 day lead times associated with custom orders
  • Research Use Only (RUO); QC-released by TriLink BioTechnologies

The ContrOligo™ PolyA RNA Standard, 100A is one size in TriLink's ContrOligo™ PolyA RNA Standards Kit, which also includes 40A, 60A, 80A, 120A, 140A standards. The full kit provides a comprehensive ladder for RNA analytical workflows.

Product Details
Catalog No. O-5001-100A
Purity >90% by analytical HPLC 
Format Lyophilized 
Recommended Storage At or below -20°C
Application HPLC or fragment analyzer size marker; Mass spectrometry method development  
Length 100 nucleotides 
Composition Poly(A) homopolymer (100-mer) 
Product Grade RUO
Shipping Temp Ambient 

Product FAQs

The ContrOligo PolyA RNA Standard is a chemically synthesized poly(A) RNA oligonucleotide of defined length, intended for use as an analytical reference standard.

This standard is designed primarily for HPLC calibration and mass spectrometry calibration in RNA analytical workflows. The 10 nmole format is ideal for HPLC applications, while the 2 nmole format is well-suited for mass spectrometry applications.

Yes. The ContrOligo PolyA RNA Standard is available individually in 40A, 60A, 80A, 100A, 120A, and 140A lengths. A full kit containing all six sizes is also available.

We recommend storing the standard at -20°C or below. To minimize freeze-thaw cycles, reconstitute and aliquot into single-use quantities on first use.

The ContrOligo PolyA Standard was assessed for its identity and purity by TriLink’s QC team. An IPRP-LCMS method confirmed the target mass (± 5 Da) while AX HPLC was utilized to determine the purity (≥ 90%).

The extinction coefficients for each ContrOligo™ PolyA RNA Standard length, calculated by the nearest-neighbor method, are provided below. Use these values with the Beer-Lambert law (A = ε × c × l) to calculate molar concentration from the absorbance at 260 nm.

Length Extinction Coefficient (L/mol/cm)
40A 483,400
60A 723,400
80A 963,400
100A 1,203,400
120A 1,443,400
140A 1,683,400

The ContrOligo PolyA RNA Standard is compatible with standard RNA handling and analysis workflows, including HPLC and mass spectrometry platforms. Handle with standard RNase-free technique.

Intellectual property

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

  • ContrOligo PolyA RNA Standard, 100A - 2 nmole (sold as nmoles)
    SKU: O-5001-100A-2NM
    $250.00
  • ContrOligo PolyA RNA Standard, 100A - 10 nmole (sold as nmoles)
    SKU: O-5001-100A-10NM
    $650.00
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